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| Title: | Three-dimensional structures of translating ribosomes by Cryo-EM. |
| Authors: | Gilbert RJ, Fucini P, Connell S, Fuller SD, Nierhaus KH, Robinson CV, Dobson CM, Stuart DI |
| Sample: | 70S E. coli ribosome translating 1 Ig domain |
| Aggregation state: | Single particle (13.4 angstroms resolution) |
 Latest update: | 2011-05-26 |
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| Processing |
| Software: | SPIDER, IMAGIC, GAP, CNS, XPLOR |
| CTF correction: | Each negative dataset |
| Resolution by author: | 13.4 Å
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| Resolution method: | FSC at 0.5 cut-off |
| Processing details: | Final maps were calculated from 8032 images from a total
of 28928 from 20 individual datasets,
with scaling in reciprocal space to
crystallographic ribosome structures and correction for
map anisotropy by B-factor weighting of amplitudes
in XPLOR with respect to a similarly-treated control
inactive ribosome. Selection of particles for inclusion in
the final maps was by correlation coefficient with respect
to the alignment model, designed to maximise nascent chain
occupancy in the selected images. |
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| Scanned images: |
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| Fitting: |
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