top_cornerHomeEMD-1063  Contact us 
Image unavailable
Title:Structure of the signal recognition particle interacting with the elongation-arrested ribosome.
Authors:Halic M, Becker T, Pool MR, Spahn CM, Grassucci RA, Frank J, Beckmann R
Sample:80S RNC-SRP complex fron canis sp.
Aggregation state:Single particle (12.0 angstroms resolution)
Red flagLatest update:2011-05-26
inner_corner
tab_top_blue.gif
bullet_white.gif  Summary
tab_bottom_blue.gif
pixel.gif
tab_top_white.gif
bullet.png Experimental details
tab_bottom_white.gif
pixel.gif
tab_top_blue.gif
bullet_white.gif  Visualization
tab_bottom_blue.gif
pixel.gif
tab_top_blue.gif
bullet_white.gif  Map information
tab_bottom_blue.gif
pixel.gif
tab_top_blue.gif
bullet_white.gif  Downloads
tab_bottom_blue.gif
pixel.gif
Sample
Sample name: 80S RNC-SRP complex fron canis sp.
Oligomeric state: one srp binds one ribosome
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Mutant Organism GO identifier InterPro identifier Virus identifier Details
1ribosome-eukaryote80S ribosome4q
2ligandmammalian SRP0.4monomerfalseq
3ligandtRNA0.025monomerfalseq
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
mg/mL
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% K ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI F30300 kVFLOOD BEAMBRIGHT FIELD2 mm1000 nm4300 nmFIELD EMISSION GUNKODAK SO163 mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
EucentricGATAN LIQUID NITROGEN°° eV K K K mrad10 e/Å2
Processing
Software:spider
Resolution by author:12.0 Å
Resolution method:FSC at 0.5 cut-off
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
μm/pixellinkOTHER
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
1RY1 The domains were separately fitted by manual docking using program O
outer_corner