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Title:Three-dimensional structure of bacteriophage T4 baseplate.
Authors:Kostyuchenko VA, Leiman PG, Chipman PR, Kanamaru S, van Raaij MJ, Arisaka F, Mesyanzhinov VV, Rossmann MG
Sample:Bacteriophage T4 baseplate-tail tube assembly
Aggregation state:Single particle (12.0 angstroms resolution)
Red flagLatest update:2011-09-23
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bullet_white.gif  Summary
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bullet.png Experimental details
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Sample
Sample name: Bacteriophage T4 baseplate-tail tube assembly
Oligomeric state: the complex has sixfold symmetry
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Mutant Organism GO identifier InterPro identifier Virus identifier Details
1proteinbaseplate-tail tube complexfalsecoliphage T4
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
75 mg/mLwaterholey carbon
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% KBlot for 2 seconds before plunging ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI/PHILIPS CM300FEG/T300 kVFLOOD BEAMBRIGHT FIELD2 mm1200 nm5000 nm4500047000FIELD EMISSION GUNKodak SO163 film mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
626 Single Tilt Cryotransfer SystemGATAN LIQUID NITROGEN0°0° eV K K K mrad25 e/Å22001-01-30
Processing
Software:SPIDER
CTF correction:each particle
Resolution by author:12.0 Å
Resolution method:FSC at 0.5 cut-off
Processing details:modified SPIDER version was used to allow reconstruction of the whole baseplate-tail tube assembly
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
1514 μm/pixel8linkimages were scanned at 7 micron per pixel and averaged 2x2 to give 14 micron per pixelZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
1PDF laplacian filtered real spacecorrelation coefficientSitus 2.0, ColoresREAL
1PDI laplacian filtered real spacecorrelation coefficientSitus 2.0, ColoresREAL
1PDJ laplacian filtered real spacecorrelation coefficientSitus 2.0, ColoresREAL
1PDL laplacian filtered real spacecorrelation coefficientSitus 2.0, ColoresREAL
1PDM laplacian filtered real spacecorrelation coefficientSitus 2.0, ColoresREAL
1PDP laplacian filtered real spacecorrelation coefficientSitus 2.0, ColoresREAL
2FL8 laplacian filtered real spacecorrelation coefficientSitus 2.0, ColoresREAL
3H3W laplacian filtered real spacecorrelation coefficientSitus 2.0, ColoresREAL
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