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Title: Molecular architecture of the multiprotein splicing factor SF3b. Authors: Golas MM, Sander B, Will CL, Luhrmann R, Stark H Source: SF3b, multiprotein splicing factor Aggregation State: singleParticle, (resolution 9.7 Angstroms)   Summary   Visualisation   Sample  Experiment   Processing   Downloads Experiment Sample Preparation: ph: Sample Conc.: Details: Staining: Sample Support Details: 7.9 0.05 20 mM Hepes, 600 mM KCl, 1.5 mM Mg2 Cl2, 5% glycerol, 0.5 mM DTT, 0.5 mM PMSF The SF3b complexes were sandwiched in 2% uranyl formate between two layers of thin carbon using a perforated carbon grid as support. After 2 min at RT, the sample was frozen in liquid nitrogen. 200 mesh copper grid Vitrification: Cryogen Name: Humidity: Temp.: Instr.: Method: Time Resolved: Details: NITROGEN Imaging Conditions: Microscope: Voltage: Illumination Mode: Imaging Mode: Cs: Defocus Min: Defocus Max: Nominal Mag: Calibrated Mag: Electron Source: Detector: Detector distance: Astigmatism: FEI/PHILIPS CM200 FEG 200 FLOOD BEAM BRIGHT FIELD 2 150 1500 50000 49500 FIELD EMISSION GUN Kodak SO163 175,000 times magnification Specimen Holder: Holder Model: Tilt Min: Tilt Max: Energy Filter: Energy Window: Temp.: Temp. min: Temp. max: Beam Tilt: Electron Dose: Other Details: Date: side entry cryo holder GATAN LIQUID NITROGEN 93 20