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Title:Nucleotide-induced conformations in the neck region of dimeric kinesin.
Authors:Skiniotis G, Surrey T, Altmann S, Gross H, Song YH, Mandelkow E, Hoenger A
Sample:rat kinesin monomer ric354 complexed to microtubule in the absence of nucleotide
Aggregation state:Helical (25 angstroms resolution)
Red flagLatest update:2011-05-26
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bullet_white.gif  Summary
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bullet.png Experimental details
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bullet_white.gif  Visualization
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Sample
Sample name: rat kinesin monomer ric354 complexed to microtubule in the absence of nucleotide
Oligomeric state: monomer
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Mutant Organism GO identifier InterPro identifier Virus identifier Details
1proteinrat kinesin0.39dimertruerattus norvegicus
2proteintubulin0.11hetero-dimerfalserattus norvegicus
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
6.80.5 mg/mLPIPES 80 mM, MgCl2 1 mM, GTP 1 mM, Taxol 20 uM, DMSO 7.5%ice-embeddedholey grids
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE%93 K ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI/PHILIPS CM200FEG/ST200 kVFLOOD BEAMBRIGHT FIELD2 mm2000 nm2500 nm38000FIELD EMISSION GUNKodak SO163 film mmwas corrected at 180,000 times mag.

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
side entryGATAN LIQUID NITROGEN0°0° eV K K K mrad5 e/Å2
Processing
Software:PHOELIX, SUPRIM
Resolution by author:25 Å
Resolution method:FSC at 0.5
Processing details:Final map from 20 averaged datasets = 10 helical tubes
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
1021 μm/pixel8linkZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
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