EMDB Home EMDB Entry EMD-1040 Contact EMDB

Title: Nucleotide-induced conformations in the neck region of dimeric kinesin. Authors: Skiniotis G, Surrey T, Altmann S, Gross H, Song YH, Mandelkow E, Hoenger A Source: rat kinesin monomer complexed to microtubules in the presence of AMP-PNP Aggregation State: helical, (resolution 25 Angstroms)   Summary   Visualisation   Sample  Experiment   Processing   Downloads Experiment Sample Preparation: ph: Sample Conc.: Details: Staining: Sample Support Details: 6.8 0.5 PIPES 80 mM, MgCl2 1 mM, GTP 1 mM, Taxol 20 uM, DMSO 7.5% ice-embedded holey grids Vitrification: Cryogen Name: Humidity: Temp.: Instr.: Method: Time Resolved: Details: ETHANE 93 Imaging Conditions: Microscope: Voltage: Illumination Mode: Imaging Mode: Cs: Defocus Min: Defocus Max: Nominal Mag: Calibrated Mag: Electron Source: Detector: Detector distance: Astigmatism: FEI/PHILIPS CM200FEG/ST 200 FLOOD BEAM BRIGHT FIELD 2 2000 2500 38000 FIELD EMISSION GUN Kodak SO163 film was corrected at 180,000 times mag. Specimen Holder: Holder Model: Tilt Min: Tilt Max: Energy Filter: Energy Window: Temp.: Temp. min: Temp. max: Beam Tilt: Electron Dose: Other Details: Date: side entry GATAN LIQUID NITROGEN 0 0 5