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Title:Nucleotide-induced conformations in the neck region of dimeric kinesin.
Authors:Skiniotis G, Surrey T, Altmann S, Gross H, Song YH, Mandelkow E, Hoenger A
Sample:rat kinesin construct rK379
Aggregation state:Helical (25 angstroms resolution)
Red flagLatest update:2011-05-26
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bullet_white.gif  Summary
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Sample
Sample name: rat kinesin construct rK379
Oligomeric state: dimer
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Mutant Organism GO identifier InterPro identifier Virus identifier Details
1proteinrat kinesin0.98dimertruerattus norvegicus
2proteintubulin0.11hetero-dimerfalserattus norvegicus
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
6.80.5 mg/mLPIPES 80 mM, MgCl2 1 mM, GTP 1 mM, Taxol 20 uM, DMSO 7.5%ice-embededholey grids
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE%93 Kself made ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI/PHILIPS CM200FEG/ST200 kVFLOOD BEAMBRIGHT FIELD2 mm2000 nm2500 nm38000FIELD EMISSION GUNKodak SO163 mmwas corrected at 180,000 times mag.

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
side-entryGATAN LIQUID NITROGEN0°0° eV95 K K K mrad5 e/Å2
Processing
Software:PHOELIX, SUPREME
Resolution by author:25 Å
Resolution method:FSC at 0.5
Processing details:Final map from 33 averaged datasets = 17 helical tubes
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
1621 μm/pixel8linkZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
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