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Title:Structure of a fast kinesin: implications for ATPase mechanism and interactions with microtubules.
Authors:Song YH, Marx A, Muller J, Woehlke G, Schliwa M, Krebs A, Hoenger A, Mandelkow E
Sample:neurospora crassa kinesin monomer
Aggregation state:Helical (25 angstroms resolution)
Red flagLatest update:2011-05-26
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bullet_white.gif  Summary
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bullet.png Experimental details
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bullet_white.gif  Visualization
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bullet_white.gif  Map information
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Sample
Sample name: neurospora crassa kinesin monomer
Oligomeric state: monomer
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Mutant Organism GO identifier InterPro identifier Virus identifier Details
1proteinneurospora crassakinesinmonomertrueneurospora crassa
2proteintubulinhetero dimerfalseneurospora crassa
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
6.90.5 mg/mLPipes 20mM, 50 mM NaCl, 5mM mgcl,1mM Mg-ATP, 20um taxol.ice-embeddedholey grids
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
PROPANE%93 Kself made ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI/PHILIPS CM120T100 kVFLOOD BEAMBRIGHT FIELD mm1500 nm2000 nm37000LAB6Kodak SO163 film mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
side entryGATAN LIQUID NITROGEN0°0° eV K K K mrad5 e/Å2
Processing
Software:Phoelix, Suprim
Resolution by author:25 Å
Resolution method:FSC at 0.5
Processing details:Final maps from 36 averaged datasets = 18 helical tubes
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
1821 μm/pixel16linkZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
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