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Title:Image reconstructions of microtubules decorated with monomeric and dimeric kinesins: comparison with x-ray structure and implications for motility.
Authors:Hoenger A, Sack S, Thormahlen M, Marx A, Muller J, Gross H, Mandelkow E
Sample:rat kinesin motor domains complexed to microtubules
Aggregation state:Helical (25 angstroms resolution)
Red flagLatest update:2011-05-26
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bullet_white.gif  Summary
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bullet.png Experimental details
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bullet_white.gif  Visualization
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bullet_white.gif  Map information
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Sample
Sample name: rat kinesin motor domains complexed to microtubules
Oligomeric state: dimer
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Mutant Organism GO identifier InterPro identifier Virus identifier Details
1proteinrat kinesin84dimertruerattus norvegicusdimeric motor domain
2proteintubulin110hetero dimerfalserattus norvegicus
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
6.80.5 mg/mLPipes 80mM, MgCl 1mM, GTP 1mM, Taxol 20uM, DMSO 5%ice-embeddedholey grids
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE%93 Kself made ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
OTHER100 kVFLOOD BEAMBRIGHT FIELD mm1000 nm1600 nm35000LAB6AGFA Scienta film mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
side-entryGATAN LIQUID NITROGEN0°0° eV95 K K K mrad5 e/Å2
Processing
Software:Phoelix, Suprim
Resolution by author:25 Å
Resolution method:FSC at 0.5
Processing details:Final maps from 20 averaged datasets = 10 helical tubes
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
1020 μm/pixel16linkEMIL 10
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
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