top_cornerHomeEMD-1025  Contact us 
Image unavailable
Title:Microscopic evidence for a minus-end-directed power stroke in the kinesin motor ncd.
Authors:Wendt TG, Volkmann N, Skiniotis G, Goldie KN, Muller J, Mandelkow E, Hoenger A
Sample:SH3 tagged Ncd296 motor fragment from Drosophila Melanogaster no nucleotide state
Aggregation state:Helical
Red flagLatest update:2011-05-26
inner_corner
tab_top_blue.gif
bullet_white.gif  Summary
tab_bottom_blue.gif
pixel.gif
tab_top_white.gif
bullet.png Experimental details
tab_bottom_white.gif
pixel.gif
tab_top_blue.gif
bullet_white.gif  Visualization
tab_bottom_blue.gif
pixel.gif
tab_top_blue.gif
bullet_white.gif  Map information
tab_bottom_blue.gif
pixel.gif
tab_top_blue.gif
bullet_white.gif  Downloads
tab_bottom_blue.gif
pixel.gif
Sample
Sample name: SH3 tagged Ncd296 motor fragment from Drosophila Melanogaster no nucleotide state
Oligomeric state: SH3ncd forms a dimer
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Mutant Organism GO identifier InterPro identifier Virus identifier Details
1proteinSH3 tagged Ncd296 dimer0.1068SH3ncd296 forms a dimertrue
2proteintubulin dimer0.1polymer of alpha beta dimersfalsebovine tubulin was purchased from Cytoskeleton Inc. (Denver,CO)
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
6.82.45 mg/mL80mM PIPES, 2mM MgCl2plunge-frozen in liquid ethanequantifoil holey grids
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE%93 Kstandard guillotine msvitrification carried out at room temperature
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI/PHILIPS CM200FEG/ST160 kVFLOOD BEAMBRIGHT FIELD1.26 mm1500 nm2000 nm38000TUNGSTEN HAIRPINKodak SO163 film mmobjective lens astigmatism was corrected at 150K mag

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
side entryGATAN LIQUID NITROGEN0°0° eV93 K K K mrad e/Å2low dose imaging
Processing
Software:PHOELIX
Processing details:final map was calculated from 27 datasets of near and far sides
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
1421 μm/pixel0.98linkZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
outer_corner