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Title: Three-dimensional reconstructions from cryoelectron microscopy images reveal an intimate complex between helicase DnaB and its loading partner DnaC. Authors: San Martin C, Radermacher M, Wolpensinger B, Engel A, Miles CS, Dixon NE, Carazo JM Source: DnaB from E.coli Aggregation State: singleParticle, (resolution 34.5 Angstroms)   Summary   Visualisation   Sample  Experiment   Processing   Downloads Experiment Sample Preparation: ph: Sample Conc.: Details: Staining: Sample Support Details: 7.6 .04 50 mM Tris.HCl pH 7.6 2 mM DTT 5 mM MgCl2 200 mM NaCl 0.25 mM ADP Vitrification: Cryogen Name: Humidity: Temp.: Instr.: Method: Time Resolved: Details: ETHANE double side blotting device samples were adsorbed onto carbon-coated molybdenum holey grids after 30s glow discharge, and vitrified by quick plunging in liquid ethane in a double-side blotting device Imaging Conditions: Microscope: Voltage: Illumination Mode: Imaging Mode: Cs: Defocus Min: Defocus Max: Nominal Mag: Calibrated Mag: Electron Source: Detector: Detector distance: Astigmatism: PHILIPS EM420 100 FLOOD BEAM BRIGHT FIELD 2 1500 2000 60000 LAB6 Kodak SO163 film Specimen Holder: Holder Model: Tilt Min: Tilt Max: Energy Filter: Energy Window: Temp.: Temp. min: Temp. max: Beam Tilt: Electron Dose: Other Details: Date: Gatan626 GATAN LIQUID NITROGEN 45 98 10