top_cornerHomeEMD-1020  Contact us 
Image unavailable
Title:Structure of a viral DNA gatekeeper at 10 A resolution by cryo-electron microscopy.
Authors:Orlova EV, Gowen B, Droge A, Stiege A, Weise F, Lurz R, van Heel M, Tavares P
Sample:Bacteriophage SPP1 portal protein gp6
Aggregation state:Single particle (10 angstroms resolution)
Red flagLatest update:2011-05-26
inner_corner
tab_top_blue.gif
bullet_white.gif  Summary
tab_bottom_blue.gif
pixel.gif
tab_top_white.gif
bullet.png Experimental details
tab_bottom_white.gif
pixel.gif
tab_top_blue.gif
bullet_white.gif  Visualization
tab_bottom_blue.gif
pixel.gif
tab_top_blue.gif
bullet_white.gif  Map information
tab_bottom_blue.gif
pixel.gif
tab_top_blue.gif
bullet_white.gif  Downloads
tab_bottom_blue.gif
pixel.gif
Sample
Sample name: Bacteriophage SPP1 portal protein gp6
Oligomeric state: isolated portal protein has 13- fold cyclical symmetry
Theoretical molecular weight of the sample: 0.0573
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Mutant Organism GO identifier InterPro identifier Virus identifier Details
1proteinBacteriophage SPP1 portal protein gp6C13falseBacilus subtilis Bacteriophage SPP1
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
7.51.5 mg/mL
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% KStandard plungerBlot about 2-3 seconds before plunging. ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI/PHILIPS CM200FEG/ST200 kVFLOOD BEAMBRIGHT FIELD2.1 mm1700 nm3000 nm5000048600FIELD EMISSION GUNKodak SO163 mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
side-entryGATAN LIQUID NITROGEN°° eV K K K mrad10 e/Å21999-01-01
Processing
Software:IMAGIC
CTF correction:CTF correction of each particle
Resolution by author:10 Å
Resolution method:FSC at 20 sigma
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
1510.2 μm/pixel1.68linkImages were digitised using a patch work densitometer.PATCHWORK DENSITOMETER
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
Angular reconstitutionMolrep
outer_corner