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EMDB Entry EMD-1019
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Title
:
The Escherichia coli large ribosomal subunit at 7.5 A resolution.
Authors
:
Matadeen R, Patwardhan A, Gowen B, Orlova EV, Pape T, Cuff M, Mueller F, Brimacombe R, van Heel M
Aggregation State
:
singleParticle, (resolution 7.5 Angstroms)
Summary
Visualisation
Sample
Experiment
Processing
Downloads
Experiment
Sample Preparation:
ph:
Sample Conc.:
Details:
Staining:
Sample Support Details:
7.4
1
20mM HEPES-KOH 6 mM MgCl2, 150 mM NH4Cl
Vitrification:
Cryogen Name:
Humidity:
Temp.:
Instr.:
Method:
Time Resolved:
Details:
ETHANE
in-house freeze plunger
Blot for 2 seconds before plunging
Imaging Conditions:
Microscope:
Voltage:
Illumination Mode:
Imaging Mode:
Cs:
Defocus Min:
Defocus Max:
Nominal Mag:
Calibrated Mag:
Electron Source:
Detector:
Detector distance:
Astigmatism:
FEI/PHILIPS CM200FEG/UT
200
FLOOD BEAM
BRIGHT FIELD
2
1000
3000
38000
37600
FIELD EMISSION GUN
Kodak SO163 film
Specimen Holder:
Holder Model:
Tilt Min:
Tilt Max:
Energy Filter:
Energy Window:
Temp.:
Temp. min:
Temp. max:
Beam Tilt:
Electron Dose:
Other Details:
Date:
eucentric
GATAN LIQUID NITROGEN
96
10
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