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Title:The first step: activation of the Semliki Forest virus spike protein precursor causes a localized conformational change in the trimeric spike.
Authors:Ferlenghi I, Gowen B, de Haas F, Mancini EJ, Garoff H, Sjoberg M, Fuller SD
Sample:Semliki forest mSQL
Aggregation state:Icosahedral (9.0 angstroms resolution)
Red flagLatest update:2011-05-26
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Sample
Sample name: Semliki forest mSQL
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Mutant Organism GO identifier InterPro identifier Virus identifier Details
1virussemliki forest m SQLsemliki forest m SQL
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
7.43 mg/mLtris(10mM) NaCL (100mM) ph 7.4
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE100%37 KEMBL plunger with warm humid air sprayblot for 2 sec Graticule grids were used to maintain ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI/PHILIPS CM200FEG/ST200 kVFLOOD BEAMBRIGHT FIELD2 mm975 nm7628 nm50000FIELD EMISSION GUNSO163 film44 mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
eucentricGATAN LIQUID NITROGEN°° eV105 K K K mrad8 e/Å2
Processing
Software:EMBL
CTF correction:ctf multiplication and summation of normalized reconstructions
Resolution by author:9.0 Å
Resolution method:FSC at 0.5 cut off
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
20 μm/pixel18linkPERKIN ELMER
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
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