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Title:A cryo-electron microscopic study of ribosome-bound termination factor RF2.
Authors:Rawat UB, Zavialov AV, Sengupta J, Valle M, Grassucci RA, Linde J, Vestergaard B, Ehrenberg M, Frank J
Sample:RF2(GGQ) wild type from E.coli
Aggregation state:Single particle
Red flagLatest update:2011-05-26
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bullet_white.gif  Summary
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bullet.png Experimental details
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bullet_white.gif  Visualization
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bullet_white.gif  Map information
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bullet_white.gif  Downloads
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Sample
Sample name: RF2(GGQ) wild type from E.coli
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Mutant Organism GO identifier InterPro identifier Virus identifier Details
1ligandRF2 (GGQ) wild typetrue
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
mg/mL
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% K msinformation is the same as in submission 1187
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI F20200 kVFLOOD BEAMBRIGHT FIELD mm nm nmFIELD EMISSION GUN mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
Oxford, Cryo-transfer 3500GATAN LIQUID NITROGEN°° eV K K K mrad e/Å2information is the same as in submission 1187
Processing
Processing details:information information is the same as in submission 1187. SPIDER was used for the computational isolation of the RF2 (GGQ) wild type cryo-density from the 3D-EM map of RF2 (wt) bound to the E.coli 70s ribosome (submission 1187).
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
μm/pixellinkZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
1M16 1MVR 
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