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split luciferase complementation

^ http://purl.obolibrary.org/obo/MI_1203


Two proteins of interest, a bait and prey, which are genetically fused to amino- and carboxy-terminal fragments of luciferase, are transiently expressed. Physical interactions of these bait and prey proteins reconstitute some of the luciferase activity and result in light emission in the presence of the luciferase substrate. [ PMID:20734273 ]

Term info

Subsets

PSI-MI_slim

created by

orchard

creation date

2012-06-07T04:56:59Z

definition

Two proteins of interest, a bait and prey, which are genetically fused to amino- and carboxy-terminal fragments of luciferase, are transiently expressed. Physical interactions of these bait and prey proteins reconstitute some of the luciferase activity and result in light emission in the presence of the luciferase substrate.

id

MI:1203

Term relations