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electrophoretic mobility shift assay

^ http://purl.obolibrary.org/obo/MI_0413


This method proves the interaction between a nucleic acid and a protein partner. On the same electrophoresis gel 1 lane is loaded with a nucleic acid of known sequence, a second lane is loaded with the same nucleic acid together with a purified protein (or a protein mixture). The nucleic acid is often radio-labelled to enable visualisation by autoradiography. Comparison of the nucleic acid migration in the two lanes enables the retardation of the nucleic acid due to its interaction with a protein to be observed. [ http://www.ncbi.nlm.nih.gov/pubmed/12169687 ]

Synonyms: emsa, Gel retardation assay, band shift