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electrophoretic mobility supershift assay

^ http://purl.obolibrary.org/obo/MI_0412


The EMSA supershift is a EMSA experiment carried out using a third lane loaded with the radiolabeled nucleic acid, a protein mixture and an antibody for a specific protein. If an extra retardation is observed, this is due to the formation of a larger complex including the antibody. By this approach, at least one protein of the complex is directly identified. [ http://www.ncbi.nlm.nih.gov/pubmed/12169687 ]

Synonyms: emsa supershift

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