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western blot

^ http://purl.obolibrary.org/obo/MI_0113


Western blot is a procedure to identify and quantify proteins. A mixture of protein is first submitted to an electrophoresis in denaturing condition and then electro-transferred from the gel to a membrane. The membrane is then incubated with a primary antibody specific for a given protein or a specific residue modification in the sample under analysis. A secondary antibody, radiolabelled or fused to fluorophore or to a chromogenic enzyme, targets the first antibody and allows the visualisation of the protein band on the membrane. [ http://www.ncbi.nlm.nih.gov/pubmed/14755292 ]

Synonyms: Immuno blot