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molecular sieving

^ http://purl.obolibrary.org/obo/MI_0071


In sizing columns (gel filtration), the elution position of a protein or of a complex depends on its Stokes radius. Molecules with a radius that is smaller than the bead size are retained and retarded by the interaction with the matrix. The observation that two proteins, loaded on a sieving column, elute in a fraction(s) corresponding to a MW that is larger than the MW of either protein may be taken as an indication that the two proteins interact. Furthermore this technique provides a conceptually simple method for evaluating the affinity of the interaction. [ http://www.ncbi.nlm.nih.gov/pubmed/7708014 ]

Synonyms: Sizing column, Gel Filtration, Size Exclusion Chromatography

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id

MI:0071

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