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two hybrid fragment pooling approach

^ http://purl.obolibrary.org/obo/MI_0399


Individual baits are mated against pools of random fragmented preys. The usage of degenerated fragment allows identification of the minimal protein region required for the interaction. Since multiple clones that encode overlapping regions of protein are often identified, the minimal domain for interaction may be readily apparent from the initial screen. [ http://www.ncbi.nlm.nih.gov/pubmed/12634794 ]

Synonyms: 2h fragment pooling

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id

MI:0399

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