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PISA is an interactive tool for the exploration of macromolecular
(protein, DNA/RNA and ligand) interfaces, prediction of probable
quaternary structures (assemblies), database searches of structurally
similar interfaces and assemblies, as well as searches on various
assembly and PDB entry parameters.
Using this service, you can
- retrieve results, precalculated for the whole
PDB archive, from PISA
database, or calculate interactively for your structure,
uploaded as a PDB or mmCIF file:
- structural and chemical properties of
macromolecular surfaces
- structural and chemical properties of
macromolecular interfaces
- probable quaternary structures (assemblies),
their structural and chemical properties and probable
dissociation pattern
- search PDB archive for particular interfaces formed by structural
homologues
- search PISA database of precalculated results using a wide range
of options, such as multimeric state, symmetry
number, space group, accessible/buried suraface area, free energy
of dissociation, presence/absence of salt bridges and disulphide
bonds, homomeric type, ligands and keywords
- assess significance (biological role) of macromolecular interfaces
- download and visualise structures, interfaces and assemblies
using Rasmol
(Unix/Linux platforms),
Rastop
(MS Windows machines) and
Jmol
(platform-independent server-side java viewer)
Please read "Disclaimer", "Tips" and "Visualisation" sections from the
following list before working with the server for the first time. Other
topics may also be useful.
PISA is based on the new CCP4 Coordinate Library, found
here.
PISA uses
SSM
technology for structural searches.
PISA queries may be launched from any web site, see instructions
here.
PISA data may be downloaded using URL links, see instructions
here.
FEEDBACK
We appreciate you using this service. We keep working on it in order
to better meet the needs of protein science community.
If the service has not met your expectations, we would be grateful
if you let us know why. Any questions, comments, suggestions and bug
reports are welcome.
Send your message
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