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  2. In curation
  3. In review
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MTBLS8:  Growth control of the eukaryote cell: a systems biology study in yeast

 Authors: Juan Castrillo , Stephen Oliver , Warwick Dunn , Neil Swainston

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  Submitted: 19-Apr-2012 , Release date: 19-Apr-2012 , Update date: 04-Dec-2015

 Study status: Public

Study Description

Metabolic control analysis is being exploited in a systems biology study of the eukaryotic cell. Using chemostat culture, we have measured the impact of changes in flux (growth rate) on the transcriptome, proteome, endometabolome and exometabolome of the yeast Saccharomyces cerevisiae. Each functional genomic level shows clear growth-rate-associated trends and discriminates between carbon-sufficient and carbon-limited conditions. Genes consistently and significantly upregulated with increasing growth rate are frequently essential and encode evolutionarily conserved proteins of known function that participate in many protein-protein interactions. In contrast, more unknown, and fewer essential, genes are downregulated with increasing growth rate; their protein products rarely interact with one another. A large proportion of yeast genes under positive growth-rate control share orthologs with other eukaryotes, including humans. Significantly, transcription of genes encoding components of the TOR complex (a major controller of eukaryotic cell growth) is not subject to growth-rate regulation. Moreover, integrative studies reveal the extent and importance of post-transcriptional control, patterns of control of metabolic fluxes at the level of enzyme synthesis, and the relevance of specific enzymatic reactions in the control of metabolic fluxes during cell growth.

  Organism(s)

Saccharomyces cerevisiae

  Study Design Description

untargeted metabolites

SBO:systems biology representation

endometabolome

exometabolome

CHMO:gas chromatography-mass spectrometry

  Experimental Factors

nutrient

growth rate

Protocol Description
Sample collection The diploid Saccharomyces cerevisiae strain FY1679 were grown in fermenters, essentially as described before [1]. A set of chemostat cultures under C-, N-, P- and S-limiting conditions were established at a series of dilution rates (0.07, 0.10, 0.20 /h). Cultures were fed with a defined mineral medium limiting growth by carbon, nitrogen, phosphorus, or sulphur, all other nutritional requirements were in excess at a constant residual concentration. Glucose and vitamins were added to the medium after separate sterilization. Glucose was heat-sterilized at 120 °C. Vitamins were prepared and sterilized by filtration through 0.2 µm filters (Sartopore®2, Sartorius, UK). Chemostat cultivation was performed in 2-litre fermenters (FT Applikon Ltd., UK) with a working volume of 1.0 l, a temperature of 30 °C and a stirrer speed of 750 rpm. The culture pH was maintained at 5.0 by automatic addition of 2 M NaOH or HCl as required, via an Applikon ADI-1030 biocontroller. Aerobic cultivation was performed by maintaining an air flow of 1.0 l/min. The dissolved oxygen concentration in all chemostat cultures, as measured with an Ingold polarographic oxygen electrode, was above 50% of saturation. To avoid loss of volatile metabolites, the off-gas condenser was cooled to 4 °C, connected to a cryostat. Off-gas oxygen and carbon dioxide levels were monitored with a Tandem gas analyser (Magellan Instruments, UK). Steady state conditions were deemed established once biomass levels (absorbance and dry weight measurements), dissolved oxygen, and off-gas fractions remained constant over three residence times (three volume changes). For the rapamycin study, a batch culture growing at mid-exponential phase in minimal medium was established under the same cultivation conditions as for chemostat experiments. This was divided into two flasks. Rapamycin (200 ng/ml) was added to one half, and the vehicle added to the other, as the control. Samples were taken at 0, 1, 2 and 4 hrs after the treatment.

Ref:
[1] Baganz F1, Hayes A, Marren D, Gardner DC, Oliver SG. Suitability of replacement markers for functional analysis studies in Saccharomyces cerevisiae. Yeast. 1997 Dec;13(16):1563-73. PMID:9509575
Extraction Sampling, quenching, and efficient extraction for endo- and exometabolome analyses of hundreds of intra- and extracellular metabolites, including the main glycolytic intermediates, nucleotides, pyridine nucleotides, and organic acids (for example, pyruvate, citrate, and succinate) were carried out as described previously [1]. Direct quenching of the culture was performed by fast sampling of a volume of culture equivalent to 30 mg dry weight; extracts and culture supernatants were stored at -80 °C. Samples were prepared for MS immediately before the analysis was carried out.

Ref:
[1] Castrillo JI, Hayes A, Mohammed S, Gaskell SJ, Oliver SG. An optimized protocol for metabolome analysis in yeast using direct infusion electrospray mass spectrometry. Phytochemistry. 2003 Mar;62(6):929-37.
Chromatography Two different sample types were analyzed, endometabolome and exometabolome. Endometabolome samples (typically 1000 µl) were spiked with 4 µl internal standard solution (2.22 mg/ml [²H2]malonic acid, 1.92 mg/ml [²H5]glycine and 0.61 mg/ml [¹³C6]glucose dissolved in water) and lyophilized using a Speedvac concentrator vacuum system SPD111V connected to a Micromodulyo Freeze Dryer (ThermoLife Sciences, Basingstoke, UK). Exometabolome samples (200 µl) were spiked with 20 µl internal standard solution and lyophilized as previously described. Dried samples were derivatized as follows; 100 µl of 20 mg/ml O-methylhydroxylamine solution was added and heated at 40 °C for 90 minutes followed by addition of 100 µl N-acetyl-N-(trimethylsilyl)-trifluoroacetamide and heating at 40 °C for 120 mins. The final solution was spiked with 20 µl retention index solution (0.6 mg/ml n-decane, n-dodecane, n-pentadecane, n-nonadecane, n-docosane dissolved in hexane).

Chromatography was carried out on Agilent 6890 gas chromatograph using the manufacturer's software (ChromaTOF version 2.12) and a DB-50 GC column (Supelco, Gillingham, UK; 30 m x 0.25 mm x 0.25 µm film thickness).
Mass spectrometry The sample was injected (1 µl) split ratio of 1 in 3 into a Agilent 6890 GC equipped with DB-50 GC column (injector temperature = 270 °C, helium carrier gas flow rate = 1 ml/min). The initial column temperature was 70 °C; this was held for 2 mins and then increased by 24 °C/min to 280 °C and hold for 5 mins. The column effluent was introduced into a LECO Pegasus III time-of-flight mass spectrometer (LECO, St Joseph, MI) with transfer line temperature = 250 °C, ion source temperature = 230 °C). The TOF mass spectrometer can collect spectra at up to 500 Hz and mass range of 30–500 m/z. In the ChromaTOF software the S/N threshold was set at 10, baseline offset at 1.0, data points for averaging at 7, and peak width at 2.5.
Data transformation In the ChromaTOF software the S/N threshold was set at 10, baseline offset at 1.0, data points for averaging at 7, and peak width at 2.5. Initial processing of raw data was undertaken using LECO ChromaTOF v2.12 software to construct a data matrix (metabolite peak versus sample number) using response ratios (peak area metabolite/peak area [²H2]malonic acid) to calculate the relative amount of each metabolite in each sample. For each metabolite peak for each set of three biological replicates, the CV was calculated as follows: %CV = (standard deviation/mean) × 100. The mean CV for replicate analytical analyses (n = 3) was 24.6% for all analyses undertaken, with 62% of these analyses having a CV less than 20% and 38.04% having one less than 10%. Although this shows precision to be less robust than for targeted analyses, this is appropriate in metabolic profiling, where many hundreds of metabolites are detected in short analysis times.
Metabolite identification Metabolite peaks were initially identified by searches on a commercially available mass spectral library [NIST/EPA/NIH (02)] (US National Institute of Standards and Technology, the Environmental Protection Agency and the National Institutes of Health; 2002) and libraries prepared by one of us (W.B.D.). For a peak to be identified required a similarity and reverse match score of greater than 700. Metabolite identification was confirmed by the analysis of pure chemical standards in identical conditions to the sample analysis. Identification was confirmed if the retention time (±5 secs) and mass spectra (similarity and reverse matches greater than 750) of metabolite peak in sample and standard were equivalent.
Source Name Organism Variant Organism part Protocol REF Sample Name nutrient growth rate
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 1_1 Carbon limiting 0.07
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 1_2 Carbon limiting 0.07
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 1_3 Carbon limiting 0.07
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 2_1 Carbon limiting 0.07
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 3_1 Carbon limiting 0.07
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 4_1 Carbon limiting 0.1
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 5_1 Carbon limiting 0.1
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 6_1 Carbon limiting 0.1
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 7_1 Carbon limiting 0.2
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 8_1 Carbon limiting 0.2
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 9_1 Carbon limiting 0.2
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 10_1 Nitrogen limiting 0.07
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 10_2 Nitrogen limiting 0.07
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 10_3 Nitrogen limiting 0.07
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 11_1 Nitrogen limiting 0.07
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 12_1 Nitrogen limiting 0.07
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 13_1 Nitrogen limiting 0.1
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 14_1 Nitrogen limiting 0.1
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 15_1 Nitrogen limiting 0.1
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 16_1 Nitrogen limiting 0.2
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 17_1 Nitrogen limiting 0.2
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 18_1 Nitrogen limiting 0.2
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 19_1 Phosphate limiting 0.07
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 19_2 Phosphate limiting 0.07
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 19_3 Phosphate limiting 0.07
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 20_1 Phosphate limiting 0.07
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 21_1 Phosphate limiting 0.07
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 22_1 Phosphate limiting 0.1
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 23_1 Phosphate limiting 0.1
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 24_1 Phosphate limiting 0.1
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 25_1 Phosphate limiting 0.2
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 26_1 Phosphate limiting 0.2
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 27_1 Phosphate limiting 0.2
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 28_4 Sulphate limiting 0.07
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 28_5 Sulphate limiting 0.07
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 28_6 Sulphate limiting 0.07
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 29_2 Sulphate limiting 0.07
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 30_1 Sulphate limiting 0.07
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 31_1 Sulphate limiting 0.1
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 32_1 Sulphate limiting 0.1
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 33_1 Sulphate limiting 0.1
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 34_1 Sulphate limiting 0.2
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 35_1 Sulphate limiting 0.2
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 36_1 Sulphate limiting 0.2
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 37_1 Ethanol limiting 0.07
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 37_2 Ethanol limiting 0.07
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 37_3 Ethanol limiting 0.07
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 38_1 Ethanol limiting 0.07
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 39_1 Ethanol limiting 0.07
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 40_1 Ethanol limiting 0.1
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 41_1 Ethanol limiting 0.1
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 42_1 Ethanol limiting 0.1
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 46_1 Glucose/O2 limiting 0.07
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 46_2 Glucose/O2 limiting 0.07
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 46_3 Glucose/O2 limiting 0.07
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 47_1 Glucose/O2 limiting 0.07
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 48_1 Glucose/O2 limiting 0.07
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 49_1 Glucose/O2 limiting 0.1
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 50_1 Glucose/O2 limiting 0.1
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 51_1 Glucose/O2 limiting 0.1
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 52_1 Glucose/O2 limiting 0.2
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 53_1 Glucose/O2 limiting 0.2
Internal/Endometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 whole organism Sample collection 54_1 Glucose/O2 limiting 0.2
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 55_1 Carbon limiting 0.07
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 55_2 Carbon limiting 0.07
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 55_3 Carbon limiting 0.07
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 56_1 Carbon limiting 0.07
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 57_1 Carbon limiting 0.07
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 58_1 Carbon limiting 0.1
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 59_1 Carbon limiting 0.1
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 60_1 Carbon limiting 0.1
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 61_1 Carbon limiting 0.2
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 62_1 Carbon limiting 0.2
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 63_1 Carbon limiting 0.2
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 64_1 Nitrogen limiting 0.07
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 64_2 Nitrogen limiting 0.07
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 64_3 Nitrogen limiting 0.07
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 65_1 Nitrogen limiting 0.07
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 66_1 Nitrogen limiting 0.07
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 67_1 Nitrogen limiting 0.1
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 68_1 Nitrogen limiting 0.1
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 69_1 Nitrogen limiting 0.1
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 70_1 Nitrogen limiting 0.2
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 71_1 Nitrogen limiting 0.2
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 72_1 Nitrogen limiting 0.2
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 73_1 Phosphate limiting 0.07
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 73_2 Phosphate limiting 0.07
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 73_3 Phosphate limiting 0.07
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 74_1 Phosphate limiting 0.07
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 75_1 Phosphate limiting 0.07
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 76_1 Phosphate limiting 0.1
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 77_1 Phosphate limiting 0.1
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 78_1 Phosphate limiting 0.1
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 79_1 Phosphate limiting 0.2
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 80_1 Phosphate limiting 0.2
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 81_1 Phosphate limiting 0.2
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 82_1 Sulphate limiting 0.07
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 82_2 Sulphate limiting 0.07
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 82_3 Sulphate limiting 0.07
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 83_1 Sulphate limiting 0.07
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 84_1 Sulphate limiting 0.07
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 85_1 Sulphate limiting 0.1
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 86_1 Sulphate limiting 0.1
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 87_1 Sulphate limiting 0.1
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 88_1 Sulphate limiting 0.2
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 89_1 Sulphate limiting 0.2
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 90_1 Sulphate limiting 0.2
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 91_1 Ethanol limiting 0.07
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 91_2 Ethanol limiting 0.07
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 91_3 Ethanol limiting 0.07
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 92_1 Ethanol limiting 0.07
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 93_1 Ethanol limiting 0.07
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 94_1 Ethanol limiting 0.1
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 95_1 Ethanol limiting 0.1
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 96_1 Ethanol limiting 0.1
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 100_1 Glucose/O2 limiting 0.07
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 100_2 Glucose/O2 limiting 0.07
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 100_3 Glucose/O2 limiting 0.07
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 101_1 Glucose/O2 limiting 0.07
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 102_1 Glucose/O2 limiting 0.07
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 103_1 Glucose/O2 limiting 0.1
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 104_1 Glucose/O2 limiting 0.1
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 105_1 Glucose/O2 limiting 0.1
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 106_1 Glucose/O2 limiting 0.2
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 107_1 Glucose/O2 limiting 0.2
External/Exometabolome Saccharomyces cerevisiae Saccharomyces cerevisiae strain FY1679 exometabolome Sample collection 108_1 Glucose/O2 limiting 0.2
Validations marked with (*) are specially approved by the MetaboLights Curators.
Click here for the detailed description of Validations.
Condition Status Description Requirement Group Message
PASSES Study Title MANDATORY STUDY OK
PASSES Study Description MANDATORY STUDY OK
PASSES Study text successfully parsed OPTIONAL STUDY OK
PASSES Study Contact(s) have listed email MANDATORY CONTACT OK
PASSES Sample(s) MANDATORY SAMPLES OK
PASSES Sample Name consistency check MANDATORY ASSAYS OK
PASSES Publication(s) associated with this Study MANDATORY PUBLICATION OK
PASSES Minimal Experimental protocol MANDATORY PROTOCOLS OK
PASSES Comprehensive Experimental protocol OPTIONAL PROTOCOLS OK
PASSES Sample Collection protocol MANDATORY PROTOCOLS OK
PASSES Protocols text successfully parsed OPTIONAL PROTOCOLS OK
PASSES Organism name MANDATORY ORGANISM OK
PASSES Organism part MANDATORY ORGANISM OK
PASSES Study Factors MANDATORY FACTORS OK
PASSES Assay platform information OPTIONAL ASSAYS OK
PASSES Assay has raw files referenced MANDATORY FILES OK
PASSES Assay referenced raw files detection in filesystem MANDATORY FILES OK
PASSES Assay(s) MANDATORY ASSAYS OK
PASSES All Assays have Metabolite Assignment File (MAF) referenced OPTIONAL FILES OK
PASSES Metabolite Assignment File (MAF) is present in Study folder MANDATORY FILES OK
PASSES Metabolite Assignment File (MAF) has correct format MANDATORY FILES OK
PASSES ISA-Tab investigation file check MANDATORY ISATAB OK

Assay 

Assay file name: a_live_mtbl8_metabolite profiling_mass spectrometry.txt
Measurement: metabolite profiling
Technology: mass spectrometry
Platform: Agilent 6890 GC - LECO Pegasus III TOF MS (LECO)

Data

Sample Name Protocol REF Post Extraction Derivatization Extract Name Protocol REF Chromatography Instrument Column model Column type Labeled Extract Name Label Protocol REF Scan polarity Scan m/z range Instrument Ion source Mass analyzer MS Assay Name Raw Spectral Data File Protocol REF Normalization Name Derived Spectral Data File Protocol REF Data Transformation Name Metabolite Assignment File
1_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 1_1 1_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
1_2 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 1_2 1_2.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
1_3 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 1_3 1_3.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
2_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 2_1 2_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
3_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 3_1 3_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
4_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 4_1 4_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
5_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 5_1 5_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
6_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 6_1 6_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
7_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 7_1 7_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
8_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 8_1 8_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
9_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 9_1 9_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
10_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 10_1 10_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
10_2 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 10_2 10_2.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
10_3 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 10_3 10_3.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
11_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 11_1 11_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
12_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 12_1 12_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
13_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 13_1 13_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
14_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 14_1 14_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
15_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 15_1 15_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
16_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 16_1 16_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
17_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 17_1 17_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
18_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 18_1 18_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
19_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 19_1 19_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
19_2 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 19_2 19_2.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
19_3 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 19_3 19_3.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
20_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 20_1 20_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
21_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 21_1 21_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
22_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 22_1 22_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
23_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 23_1 23_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
24_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 24_1 24_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
25_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 25_1 25_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
26_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 26_1 26_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
27_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 27_1 27_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
28_4 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 28_4 28_4.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
28_5 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 28_5 28_5.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
28_6 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 28_6 28_6.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
29_2 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 29_2 29_2.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
30_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 30_1 30_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
31_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 31_1 31_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
32_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 32_1 32_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
33_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 33_1 33_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
34_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 34_1 34_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
35_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 35_1 35_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
36_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 36_1 36_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
37_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 37_1 37_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
37_2 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 37_2 37_2.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
37_3 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 37_3 37_3.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
38_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 38_1 38_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
39_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 39_1 39_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
40_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 40_1 40_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
41_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 41_1 41_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
42_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 42_1 42_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
46_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 46_1 46_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
46_2 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 46_2 46_2.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
46_3 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 46_3 46_3.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
47_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 47_1 47_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
48_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 48_1 48_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
49_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 49_1 49_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
50_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 50_1 50_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
51_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 51_1 51_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
52_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 52_1 52_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
53_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 53_1 53_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
54_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 54_1 54_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
55_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 55_1 55_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
55_2 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 55_2 55_2.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
55_3 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 55_3 55_3.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
56_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 56_1 56_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
57_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 57_1 57_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
58_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 58_1 58_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
59_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 59_1 59_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
60_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 60_1 60_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
61_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 61_1 61_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
62_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 62_1 62_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
63_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 63_1 63_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
64_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 64_1 64_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
64_2 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 64_2 64_2.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
64_3 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 64_3 64_3.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
65_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 65_1 65_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
66_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 66_1 66_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
67_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 67_1 67_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
68_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 68_1 68_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
69_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 69_1 69_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
70_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 70_1 70_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
71_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 71_1 71_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
72_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 72_1 72_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
73_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 73_1 73_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
73_2 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 73_2 73_2.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
73_3 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 73_3 73_3.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
74_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 74_1 74_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
75_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 75_1 75_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
76_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 76_1 76_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
77_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 77_1 77_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
78_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 78_1 78_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
79_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 79_1 79_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
80_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 80_1 80_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
81_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 81_1 81_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
82_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 82_1 82_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
82_2 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 82_2 82_2.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
82_3 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 82_3 82_3.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
83_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 83_1 83_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
84_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 84_1 84_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
85_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 85_1 85_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
86_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 86_1 86_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
87_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 87_1 87_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
88_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 88_1 88_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
89_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 89_1 89_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
90_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 90_1 90_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
91_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 91_1 91_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
91_2 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 91_2 91_2.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
91_3 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 91_3 91_3.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
92_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 92_1 92_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
93_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 93_1 93_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
94_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 94_1 94_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
95_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 95_1 95_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
96_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 96_1 96_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
100_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 100_1 100_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
100_2 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 100_2 100_2.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
100_3 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 100_3 100_3.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
101_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 101_1 101_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
102_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 102_1 102_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
103_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 103_1 103_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
104_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 104_1 104_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
105_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 105_1 105_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
106_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 106_1 106_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
107_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 107_1 107_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv
108_1 Extraction 4/20 µl (endo/exometabolome) internal standard solution added and lyophilized sylilation Chromatography Agilent 6890 GC DB-50 GC (0.25 μm, 0.25 mm x 30 m; Supelco) medium polarity Mass spectrometry positive 30-500 LECO Pegasus III electron ionization time-of-flight 108_1 108_1.cdf Data transformation LIVE JIC big experiment GCMS data for Reza_March2012.xlsx Metabolite identification m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv

Pathways - Assay 



MetExplore Pathways Mapping

Name DB Identifier Mapped Metabolite(s) p value
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List of study files

File
audit
100_1.cdf
100_2.cdf
100_3.cdf
101_1.cdf
102_1.cdf
103_1.cdf
104_1.cdf
105_1.cdf
106_1.cdf
107_1.cdf
108_1.cdf
10_1.cdf
10_2.cdf
10_3.cdf
11_1.cdf
12_1.cdf
13_1.cdf
14_1.cdf
15_1.cdf
16_1.cdf
17_1.cdf
18_1.cdf
19_1.cdf
19_2.cdf
19_3.cdf
1_1.cdf
1_2.cdf
1_3.cdf
20_1.cdf
21_1.cdf
22_1.cdf
23_1.cdf
24_1.cdf
25_1.cdf
26_1.cdf
27_1.cdf
28_4.cdf
28_5.cdf
28_6.cdf
29_2.cdf
2_1.cdf
30_1.cdf
31_1.cdf
32_1.cdf
33_1.cdf
34_1.cdf
35_1.cdf
36_1.cdf
37_1.cdf
37_2.cdf
37_3.cdf
38_1.cdf
39_1.cdf
3_1.cdf
3MetabFiles 9August2006 for referees.xls
40_1.cdf
41_1.cdf
42_1.cdf
46_1.cdf
46_2.cdf
46_3.cdf
47_1.cdf
48_1.cdf
49_1.cdf
4_1.cdf
50_1.cdf
51_1.cdf
52_1.cdf
53_1.cdf
54_1.cdf
55_1.cdf
55_2.cdf
55_3.cdf
56_1.cdf
57_1.cdf
58_1.cdf
59_1.cdf
5_1.cdf
60_1.cdf
61_1.cdf
62_1.cdf
63_1.cdf
64_1.cdf
64_2.cdf
64_3.cdf
65_1.cdf
66_1.cdf
67_1.cdf
68_1.cdf
69_1.cdf
6_1.cdf
70_1.cdf
71_1.cdf
72_1.cdf
73_1.cdf
73_2.cdf
73_3.cdf
74_1.cdf
75_1.cdf
76_1.cdf
77_1.cdf
78_1.cdf
79_1.cdf
7_1.cdf
80_1.cdf
81_1.cdf
82_1.cdf
82_2.cdf
82_3.cdf
83_1.cdf
84_1.cdf
85_1.cdf
86_1.cdf
87_1.cdf
88_1.cdf
89_1.cdf
8_1.cdf
90_1.cdf
91_1.cdf
91_2.cdf
91_3.cdf
92_1.cdf
93_1.cdf
94_1.cdf
95_1.cdf
96_1.cdf
9_1.cdf
i_Investigation.txt
JIC Info identifications 19092006.xls
s_live_mtbl8.txt
LIVE JIC big experiment GCMS data for Reza_March2012.xlsx
a_live_mtbl8_metabolite profiling_mass spectrometry.txt
a_live_mtbl8_metabolite profiling_mass spectrometry_maf.csv
m_live_mtbl8_metabolite profiling_mass spectrometry_v2_maf.tsv

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