spacer
EBI Databases Nucleotide Databases IPD MHC Non-Human Primates (NHP)

IPD - MHC Database

Requirements for Inclusion of Sequences into the Non-Human Primate (NHP) Database

  1. Where a sequence is obtained from cDNA or where PCR products are subcloned prior to sequencing, several clones should have been sequenced.
  2. Sequencing should always be performed in both directions.
  3. If direct sequencing of PCR amplified material is performed, products from at least two separate PCR reactions should have been sequenced.
  4. An accession number in a databank must have been obtained or pending. Without an accession number we are not able to release your data on the website. Sequences may be submitted to these databases online at the following addresses:
    EMBL: /Submissions/index.html
    GenBank: http://www.ncbi.nlm.nih.gov/Genbank/index.html
    DDBJ: http://www.ddbj.nig.ac.jp/sub-e.html
  5. Full length sequences are preferable though not essential; the minimum requirements are exons 2 and 3 for an MHC class I sequence and exon 2 for an MHC class II sequence.
  6. Where a novel sequence differs only within an intron or other non-coding part of the gene, a full length sequence must be obtained, which covers all coding and non-coding regions. In the absence of a full length genomic sequence from the most closely related allele, it may be required that this also be sequenced and submitted before a name can be assigned to the novel sequence.
  7. Where possible, a paper in which the new sequence is described should be submitted for publication. Draft publications can submitted to the database by e-mail (ipdmhc.nhp@bprc.nl) or fax (+31 15 284 399).
  8. Submission of a sequence to the Nomenclature Committee should be performed using the online tool available at /imgt/hla/subs/submit.html. Researchers will be expected to complete the following questionnaire relating to the sequence and provide a comparison of their new sequence with known related alleles. If the sequence cannot be submitted using the online web tools researchers should contact ipdmhc.nhp@bprc.nl directly for details of alternative submission methods.

Further Information

For more information on the NHP nomenclature please contact ipdmhc.nhp@bprc.nl.For information regarding the website and technical issues please contact IPD Support.

 

spacer
spacer