Family

UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110kDa subunit (IPR037919)

Short name: OGT

Overlapping homologous superfamilies

None.

Family relationships

None.

Description

The UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase (OGT; EC:2.4.1.255) is a heterotrimer of one 78 kDa subunit and two 110 kDa subunits. OGT catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in a protein. Substrate proteins include histone H2B, AKT1, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1. The consequences of this form of glycosylation are diverse, including insulin resistance in muscle and adipocyte cells (brought about by inhibiting the 'Thr-308' phosphorylation of AKT1) [PMID: 20018868]; regulation of glycolysis by inhibiting PFKL activity [PMID: 22923583]; in the cell cycle O-glycosylation stabilizes ARNTL/BMAL1 and CLOCK, preventing their ubiquitination and subsequent degradation [PMID: 23395176]; glycosylation of HCFC1 and interaction with TET proteins promotes binding of the SET1/COMPASS methyltransferase SETD1A to chromatin [PMID: 23353889]; and H2B GlcNAcylation is a histone modification that facilitates H2BK120 monoubiquitination [PMID: 22121020]. It is a component of several complexes, including MLL5-L, NSL [PMID: 20018852] and THAP1/THAP3-HCFC1-OGT [PMID: 20200153].

This entry represents the 110 kDa subunit which has thirteen tetratricopeptide (TPR) repeats that are required for substrate binding and oligomerization [PMID: 15361863].

The NSL complex is involved in acetylation of nucleosomal histone H4 on several lysine residues and therefore may be involved in the regulation of transcription. The complex is composed of at least MOF/KAT8, KANSL1, KANSL2, KANSL3, MCRS1, PHF20, OGT1/OGT, WDR5 and HCFC1 [PMID: 20018852].

GO terms

Biological Process

GO:0006493 protein O-linked glycosylation

Molecular Function

GO:0016757 transferase activity, transferring glycosyl groups

Cellular Component

No terms assigned in this category.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.
PANTHER