Homologous Superfamily

Exotoxin A, middle domain superfamily (IPR036478)

Short name: Exotox-A_middle_dom_sf


This domain, found in Pseudomonas aeruginosa exotoxin A, is responsible for transmembrane targeting of the toxin, as well as transmembrane translocation of the catalytic domain into the cytoplasmic compartment. A furin cleavage site is present within the domain: cleavage generates a 37 kDa carboxy-terminal fragment, which includes the enzymatic domain, which is then is translocated into the cytoplasm. It adopts a helical structure, with six alpha-helices forming a bundle [PMID: 11734000].

Proteins contain this domain include exotoxin A from Pseudomonas aeruginosa and cholix toxin from Vibrio cholerae [PMID: 18276581]. They are NAD-dependent ADP-ribosyltransferases (ADPRT) that catalyses the transfer of the ADP-ribosyl moiety of oxidized NAD onto eukaryotic elongation factor 2 (eEF-2) thus arresting protein synthesis [PMID: 18276581].

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.