Pathways & interactions
HIT-like superfamily (IPR036265)
Short name: HIT-like_sf
- Histidine triad (HIT) protein (IPR001310)
- Galactose-1-phosphate uridyl transferase, class I (IPR001937)
- CDP-diacylglycerol pyrophosphatase (IPR003763)
- Cwf19-like, C-terminal domain-1 (IPR006768)
- Scavenger mRNA decapping enzyme DcpS/DCS2 (IPR008594)
- ATP adenylyltransferase (IPR009163)
- HIT-like domain (IPR011146)
- Galactose-1-phosphate uridylyltransferase, short form, putative (IPR012361)
- CDP-diacylglycerol pyrophosphatase, proteobacterial (IPR015993)
- Histidine triad hydrolase, Hint-type (IPR026026)
- Aprataxin (IPR026963)
- Domain of unknown function DUF4921 (IPR032576)
- CDP-diacylglycerol pyrophosphatase superfamily (IPR038433)
- FHIT family (IPR039383)
- HINT family (IPR039384)
The histidine triad motif (HIT) is related to the sequence H-phi-H-phi-H-phi-phi (where phi is a hydrophobic amino acid). Proteins containing HIT domains form a superfamily of nucleotide hydrolases and transferases that act on the alpha-phosphate of ribonucleotides [PMID: 12119013]. HIT-containing proteins fall into three families:
- Hint family of protein kinase-interacting proteins. These include adenosine 5'-monophosphoramide hydrolases (e.g. HIT-nucleotide-binding protein, or HINT) and diadenosine polyphosphate hydrolases (e.g. fragile HIT protein, or FINT). These HIT domains have a topology similar to that found in the N-terminal of protein kinases [PMID: 15904496].
- GalT family. These include specific nucleoside monophosphate transferases (e.g. galactose-1-phosphate uridylyltransferase, diadenosine tetraphosphate phosphorylase, and adenylyl sulphate:phosphate adenylytransferase). These HIT domains are a duplication consisting of 2 HIT-like motifs. This family binds zinc and iron.
- mRNA decapping enzyme family. These include enzymes such as DcpS and Dcp2. The HIT-domain is usually C-terminal in these proteins [PMID: 15273322].