Topoisomerase 6 subunit A/Spo11, TOPRIM domain (IPR034136)

Short name: TOPRIM_Topo6A/Spo11

Overlapping homologous superfamilies

Domain relationships



This topoisomerase-primase (TOPRIM) nucleotidyl transferase/hydrolase domain is found in the type II topoisomerase VIA and Spo11. This subgroup contains proteins similar to Sulfolobus shibatae topoisomerase VIA (TopoVIA) [PMID: 7961685] and Saccharomyces cerevisiae meiotic recombination factor Spo11 [PMID: 11413012]. Type II DNA topoisomerases catalyze the ATP-dependent transport of one DNA duplex through another, in the process generating transient double strand breaks via covalent attachments to both DNA strands at the 5' positions. TopoVI enzymes are heterotetramers found in archaea and plants [PMID: 12618182, PMID: 11410368]. Spo11 plays a role in generating the double strand breaks that initiate homologous recombination during meiosis [PMID: 11809802, PMID: 12437782]. S. shibatae TopoVI relaxes both positive and negative supercoils, and in addition has a strong decatenase activity [PMID: 10545127].

The TOPRIM domain has two conserved motifs, one of which centres at a conserved glutamate and the other one at two conserved aspartates (DxD). For topoisomerases, the conserved glutamate is believed to act as a general base in strand joining, and as a general acid in strand cleavage [PMID: 9722641]. The DXD motif may co-ordinate Mg2+, a cofactor required for full catalytic function.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.