CRISPR-associated endonuclease Cas9, PAM-interacting domain (IPR032237)

Short name: Cas9_PI

Overlapping homologous superfamilies


Domain relationships



This entry represents a domain found in the C-terminal of bacterial type II CRISPR system Cas9 endonuclease. This domain adopts a novel protein fold that is unique to the Cas9 family. It is positioned in the structure-DNA-complex to recognise the PAM sequence on the non-complementary DNA strand of the crRNA. PAM sequence is protospacer-adjacent motifs on DNA. Cas9 carries two nuclease domains, HNH and RuvC, which cleave the DNA strands that are complementary and non-complementary to the 20 nucleotide guide sequence in crRNAs, respectively [PMID: 24634220].

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.