Domain

Krueppel-like factor 1, transactivation domain 1 (IPR031786)

Short name: EKLF_TAD1

Overlapping homologous superfamilies

None.

Domain relationships

None.

Description

This is the first part of the minimal transactivation (TAD) domain of of krueppel-like factor 1 (also known as EKLF), a transcription regulator of erythrocyte development that probably serves as a general switch factor during erythropoiesis. At the first 90 residues at the N terminus, EKLF carries a minimal TAD domain that is highly acidic. This minimal TAD of EKLF can be further subdivided into two independent domains, TAD1 (residues 1-40) and TAD2 (residues 51-90), that are both capable of independently activating transcription. TAD1 is able to form a non-covalent interaction with ubiquitin. Both TAD1 and TAd2 are highly acidic and carry a PEST (sequence rich in proline, glutamic acid, serine, and threonine) region. Deletion of either PEST domain significantly slows down degradation of EKLF by ubiquitin. The minimal TAD has an overlapping activation/degradation function that is critical for the role of EKLF in red blood cell development [PMID: 24139988].

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.
Pfam