MICAL-like protein (IPR028563)

Short name: MICAL-L

Overlapping homologous superfamilies


Family relationships



MICAL (molecule Interacting with CasL) family is a group of multifunctional proteins that contain the calponin homology (CH), a LIM and a coiled-coil (CC) domains [PMID: 11827972]. They interact with receptors on the target cells, help recruiting other proteins, and promote the modulation of their activity with respect to the downstream events [PMID: 23535333]. There is only one MICAL protein found in Drosophila [PMID: 12110185], while there are 5 MICAL (MICAL1/2/3, MICAL-like1/2) isoforms found in vertebrates [PMID: 23535333]. Drosophila MICAL and vertebrate MICAL1/2/3 contain an extra N-terminal FAD (flavin adenine dinucleotide binding monooxygenase) domain, whose structure resembles that of a flavo-enzyme, p-hydroxybenzoate hydroxylase [PMID: 16275926]. Drosophila MICAL has an NADPH-dependent actin depolymerising activity [PMID: 23535333]. Vertebrate MICALs are also shown to be effectors of small Rab GTPases, which play important roles in vesicular trafficking [PMID: 21537482].

MICAL-like protein 1 (MICAL-L1) interacts with small G proteins and regulates endocytic recycling of receptors [PMID: 21951725, PMID: 20585517]. It forms a complex with Rab13 that regulates EGFR trafficking at late endocytic pathways [PMID: 21795389]. MICAL-L1 also forms a complex with Arf6 that regulates Rab8a function. MICAL-L1 can be regulated by Rab35 [PMID: 21951725].

MICAL-like protein 2 (MICAL-L2, also known as JRAB) interacts with Rab13 [PMID: 18413246] and Rab8 to regulate the endocytic recycling of occludin, claudin and E-cadherin to the plasma membrane. It may thereby regulate the establishment of tight junctions and adherens junctions [PMID: 18094055]. MICAL-L2/JRAB also regulates the reorganisation of the actin cytoskeleton through interactions with actinin-1, actinin-4, and filamentous actin [PMID: 23100251], and via filamins during cell spreading [PMID: 23890175].

GO terms

Biological Process

GO:0032456 endocytic recycling

Molecular Function

GO:0017137 Rab GTPase binding

Cellular Component

No terms assigned in this category.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.