Homologous Superfamily

Homing endonuclease (IPR027434)

Short name: Homing_endonucl

Overlapping entries


Homing endonucleases are rare-cutting enzymes encoded by inteins and introns. They are found inserted within host genes in eukaryotes, bacteria, archaebacteria and viruses [PMID: 10754232]. By making a site-specific double-strand break in the intronless or inteinless alleles, these nucleases create recombinogenic ends which engage in a gene conversion process that duplicates the intron or intein [PMID: 9254693, PMID: 10487208]. There are four families of homing endonucleases classified by the conserved sequence motifs LAGLIDADG, GIY-YIG, H-N-H and His-Cys box [PMID: 9254693]. Endonucleases of the DOD family contain one or two copies of the 10-residue sequence known as a dodecapeptide or LAGLIDADG motif. LAGLIDADG endonucleases are either monomers, such as I-SceI, that are composed of two pseudosymmetric subdomains, or homodimers, such as I-CreI. In both cases, the LAGLIDADG endonuclease folds into a beta-saddle architecture, a common motif for nucleic acid binding proteins [PMID: 18424798].

This superfamily represents the homing endonuclease domain.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.