Microtubule-associated protein RP/EB (IPR027328)

Short name: MAPRE

Overlapping homologous superfamilies


Family relationships


This entry represents the microtubule-associated protein RP/EB (MAPRE) family, including MAPRE1 (EB1), MAPRE2 (RP1, also known as EB2), MAPRE3 (EBF3, also known as EB3) and their homologues from eukaryotes. Despite their high protein sequence conservation, the individual EBs exhibit different regulatory and functional properties [PMID: 20008324]. For instance, EB1 and EB3, but not EB2, promote persistent microtubule growth by suppressing catastrophes [PMID: 19255245].

EB1 contains an N-terminal calponin homology (CH) domain that is responsible for the interaction with microtubules (MTs), and a C-terminal coiled coil domain that extends into a four-helix bundle, required for dimer formation [PMID: 18322465]. Through their C-terminal sequences, EBs interact with most other known +TIPs (plus end tracking proteins) and recruit many of them to the growing MT ends [PMID: 12388762, PMID: 18322465]. EB1 is involved in MT anchoring at the centrosome and cell migration [PMID: 15311282].

EB2 is highly expressed in pancreatic cancer cells, and seems to be involved in perineural invasion [PMID: 19787265].

EB3 is specifically upregulated upon myogenic differentiation. Knockdown of EB3, but not that of EB1, prevents myoblast elongation and fusion into myotubes [PMID: 17658256].

GO terms

Biological Process

No terms assigned in this category.

Molecular Function

GO:0008017 microtubule binding

Cellular Component

No terms assigned in this category.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.