Active Site

Ubiquitin-conjugating enzyme, active site (IPR023313)

Short name: UBQ-conjugating_AS


Ubiquitin-conjugating enzymes (EC:, UBC or E2 enzymes) catalyze the covalent attachment of ubiquitin to target proteins. An activated ubiquitin moiety is transferred from an ubiquitin-activating enzyme (E1) to E2 which later ligates ubiquitin directly to substrate proteins with or without the assistance of 'N-end' recognizing proteins (E3) [PMID: 2193438, PMID: 1647207, PMID: 1656558]. In most species there are many forms of UBC (at least 9 in yeast) which are implicated in diverse cellular functions.

A cysteine residue is required for ubiquitin-thiolester formation. There is a single conserved cysteine in UBC's and the region around that residue is conserved in the sequence of known UBC isozymes. The signature pattern, of this entry, contains the active-site cysteine and spans the complete catalytic domain.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.
PROSITE patterns