XRN2-binding (XTBD) domain (IPR021859)

Short name: XTBD

Overlapping homologous superfamilies


Domain relationships



XRN2 is an essential eukaryotic exoribonuclease that processes and degrades various substrates. The ~80-residue XRN2-binding domain (XTBD) constitutes an XRN2-binding module that is employed by different metazoan proteins to link to XRN2 [PMID: 24462208, PMID: 26779609], such as:

  • Caenorhabditis elegans Partner of Xrn-Two protein 1, or PAXT-1 for short. Plays a role in maintenance of steady-state concentration and turnover of microRNAs (miRNA) by degradation of mature miRNA in complex with the exoribonuclease XRN-2 [PMID: 24462208].
  • Mammalian CDKN2A-interacting protein (CDKN2AIP) or Collaborator of ARF (CARF) that regulates DNA damage response in a dose-dependent manner through a number of signaling pathways involved in cell proliferation, apoptosis [PMID: 15109303, PMID: 24825908].
  • Mammalian CDKN2AIP N-terminal-like protein (CDKN2AIPNL).

The XTBD domain folds into a globular four-helix bundle (H1-H4) connected by three loops (L1-L3). H1-H3 form an antiparallel helical array and H4 folds back on top of H2 and H3 at a 90degree angle. The four-helical bundle is mainly stabilized by hydrophobic helix-helix interactions together with additional polar interactions between side chains located on neighbouring helices. The four-helix bundle of XTBD represents a structurally unique arrangement for XRN2 binding [PMID: 26779609].

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.
PROSITE profiles