Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain (IPR020631)

Short name: THF_DH/CycHdrlase_NAD-bd_dom

Overlapping homologous superfamilies

Domain relationships


Enzymes that participate in the transfer of one-carbon units require the coenzyme tetrahydrofolate (THF). Various reactions generate one-carbon derivatives of THF, which can be interconverted between different oxidation states by methylene-THF dehydrogenase (EC:, methenyl-THF cyclohydrolase (EC: and formyl-THF synthetase (EC: [PMID: 2541774, PMID: 8485162]. The dehydrogenase and cyclohydrolase activities are expressed by a variety of multifunctional enzymes, including the tri-functional eukaryotic C1-tetrahydrofolate synthase [PMID: 2541774]; a bifunctional eukaryotic mitochondrial protein; and the bifunctional Escherichia coli folD protein [PMID: 2541774, PMID: 8485162]. Methylene-tetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclo-hydrolase share an overlapping active site [PMID: 2541774], and as such are usually located together in proteins, acting in tandem on the carbon-nitrogen bonds of substrates other than peptide bonds.

This entry represents the NAD(P)-binding domain found in these enzymes.

GO terms

Biological Process

No terms assigned in this category.

Molecular Function

GO:0004488 methylenetetrahydrofolate dehydrogenase (NADP+) activity

Cellular Component

No terms assigned in this category.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.
  • cd01080 (NAD_bind_m-THF_DH_Cyclohyd)