AT hook-like (IPR020478)

Short name: AT_hook-like

Family relationships


High mobility group (HMG)-I proteins bind preferentially to the minor groove of A.T-rich regions in double-stranded DNA [PMID: 16928331, PMID: 8414980]. DNA-binding of these, and several related, proteins is effected by an 11-residue domain known as an A.T-hook [PMID: 16928331].

Within known HMG-I proteins are found three highly conserved regions, closely related to the consensus sequence TPKRPRGRPKK [PMID: 16928331]. A synthetic oligopeptide with this sequence specifically binds to substrate DNA in a manner reminiscent of intact HMG-I proteins. Structure predictions suggest that the peptide has a secondary structure similar to the anti-tumour and anti-viral drugs netropsin and distamycin [PMID: 16928331]. These ligands, which also preferentially bind to A.T-rich DNA, effectively compete with both the synthetic peptide and the HMG-I proteins for DNA binding [PMID: 16928331]. The peptide also contains novel structural features such as a predicted Asx bend, or "hook", at its N terminus, and laterally-projecting cationic Arg/Lys "bristles", which may play a role in the binding of HMG-I proteins [PMID: 16928331]. The predicted peptide structure, the A.T-hook, is a previously undescribed DNA-binding motif [PMID: 16928331].

This family represents A.T-hook-like proteins.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.