Conserved Site

Glycoside hydrolase, family 4, conserved site (IPR019802)

Short name: GlycHydrolase_4_CS


O-Glycosyl hydrolases (EC:3.2.1.) are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families [PMID: 7624375, PMID: 8535779]. This classification is available on the CAZy (CArbohydrate-Active EnZymes) web site.

Glycoside hydrolase family 4 GH4 comprises enzymes with several known activities; 6-phospho-beta-glucosidase (EC:; 6-phospho-alpha-glucosidase (EC:; alpha-galactosidase (EC:

These enzymes require NAD and a divalent ion for their activity and are proteins of about 50 kDa; e.g. 6-phospho-alpha-glucosidase requires both NAD(H) and divalent metal (Mn2+, Fe2+, Co2+, or Ni2+) for activity [PMID: 9765262]. The signature pattern is located to a conserved region located in the central section. This region does not contain residues directly shown to be important for the catalytic activity.

GO terms

Biological Process

GO:0005975 carbohydrate metabolic process

Molecular Function

GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds

Cellular Component

No terms assigned in this category.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.
PROSITE patterns