Domain

Ubiquitin-activating enzyme, catalytic cysteine domain (IPR019572)

Short name: UBA_E1_Cys

Domain relationships

None.

Description

Ubiquitin-activating enzyme (E1 enzyme) activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thiolester and free AMP. Later the ubiquitin moiety is transferred to a cysteine residue on one of the many forms of ubiquitin-conjugating enzymes (E2) [PMID: 1986373]. This domain carries the last of five conserved cysteines that is part of the active site of the enzyme, responsible for ubiquitin thiolester complex formation, the active site being represented by the sequence motif PICTLKNFP [PMID: 11004499]. Not all proteins in this entry contain a functional active site.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.
Pfam