Checkpoint protein Rad17/Rad24, fungi/metazoa (IPR018324)
Short name: Rad17/Rad24_fun/met
Overlapping homologous superfamilies
- Checkpoint protein Rad17/Rad24 (IPR004582)
- Checkpoint protein Rad17/Rad24, fungi/metazoa (IPR018324)
This entry consists of the checkpoint protein Rad24 from budding yeast and its homologue, Rad17, from other organisms. This entry does not include Rad17 from plants.
In Saccharomyces cerevisiae, Rad24 forms a complex with replication factor C (RFC) proteins, including Rfc2, Rfc3, Rfc4, and Rfc5. When DNA damage is detected, the Rad24-RFC complex loads Rad17-Mec3-Ddc1 complex onto chromatin and activates DNA damage checkpoint, which then leads to cell cycle arrest and DNA repair [PMID: 12604797]. The Rad24-RFC complex is involved in both the mitotic and meiotic checkpoints [PMID: 10511543]. Besides checkpoint activation, Rad24 is also involved in double-strand break ends processing, DNA repair and telomere maintenance [PMID: 12944484, PMID: 11267834, PMID: 10675560, PMID: 15454530].
In human, the comparable DNA damage checkpoint components, Rad17 and the Rad1-Rad9-Hus1 (9-1-1) complex, play similar roles in DNA damage surveillance and checkpoint activation as their counter partners (Rad24, Rad17-Mec3-Ddc1) in budding yeast. Rad17 participates in the recruitment of the 9-1-1 complex onto chromatin. Besides checkpoint activation, Rad17 may also serve as a sensor of DNA replication progression, and may be involved in homologous recombination [PMID: 15297881]. Overexpression of Rad17 has been associated with human breast and colon cancers [PMID: 10232579, PMID: 11555598].
It's worth noting that the name, Rad17, has been used for different proteins in budding yeast and other organisms. In this entry, Rad17, is the homologue of the budding yeast Rad24 and has no homology with budding yeast Rad17
- TIGR00602 (rad24)