Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, subunit B /E (IPR017959)

Short name: Asn/Gln-tRNA_amidoTrfase_suB/E

Overlapping homologous superfamilies

Family relationships


Glutamyl-tRNA(Gln) amidotransferase (Gat; EC:6.3.5) provides a means of producing correctly charged Gln-tRNA(Gln) through the transamidation of mis-acylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase [PMID: 9342321]. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The enzyme is composed of three subunits: A (an amidase), B and C. It also exists in eukaryotes as a protein targeted to the mitochondria.

The heterotrimer GatABC is involved in converting Glu to Gln and/or Asp to Asn, when the amino acid is attached to the appropriate tRNA. In Lactobacillus, GatABC is responsible for producing tRNA(Gln). In Archaea, GatABC is responsible for producing tRNA(Asn), while GatDE is responsible for producing tRNA(Gln). In lineages that include Thermus, Chlamydia, or Acidithiobacillus, the GatABC complex catalyses both tRNA(Gln) and tRNA(Asn).

This entry represents aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B and glutamyl-tRNA(Gln) amidotransferase subunit E.

GO terms

Biological Process

No terms assigned in this category.

Molecular Function

GO:0016884 carbon-nitrogen ligase activity, with glutamine as amido-N-donor

Cellular Component

No terms assigned in this category.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.