Ribonuclease H1, eukaryote (IPR017067)

Short name: RNase_H1_euk

Overlapping homologous superfamilies


Family relationships



This entry represents ribonuclease H1 (RNase H1; EC: from eukaryotes. RNase H1 specifically degrades the RNA of RNA-DNA hybrids through endonucleolytic cleavage to 5'-phosphomonoester in the presence of magnesium. Human RNase H1 has a DNA-binding channel that, together with the conserved phosphate-binding pocket, confers specificity for the B form and 2'-deoxy form of DNA; the RNA strand is recognised by four consecutive 2'-OH groups and cleaved by a two-metal ion mechanism [PMID: 17964265].

In the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe, this enzyme is named ribonuclease H (RNH1).

GO terms

Biological Process

No terms assigned in this category.

Molecular Function

GO:0004523 RNA-DNA hybrid ribonuclease activity
GO:0000287 magnesium ion binding

Cellular Component

No terms assigned in this category.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.