Pathways & interactions
Signal transduction histidine kinase, sporulation regulator SpoOB (IPR016120)
Short name: Sig_transdc_His_kin_SpoOB
Two-component signal transduction systems enable bacteria to sense, respond, and adapt to a wide range of environments, stressors, and growth conditions [PMID: 16176121]. Some bacteria can contain up to as many as 200 two-component systems that need tight regulation to prevent unwanted cross-talk [PMID: 18076326]. These pathways have been adapted to response to a wide variety of stimuli, including nutrients, cellular redox state, changes in osmolarity, quorum signals, antibiotics, and more [PMID: 12372152]. Two-component systems are comprised of a sensor histidine kinase (HK) and its cognate response regulator (RR) [PMID: 10966457]. The HK catalyses its own auto-phosphorylation followed by the transfer of the phosphoryl group to the receiver domain on RR; phosphorylation of the RR usually activates an attached output domain, which can then effect changes in cellular physiology, often by regulating gene expression. Some HK are bifunctional, catalysing both the phosphorylation and dephosphorylation of their cognate RR. The input stimuli can regulate either the kinase or phosphatase activity of the bifunctional HK.
A variant of the two-component system is the phospho-relay system. Here a hybrid HK auto-phosphorylates and then transfers the phosphoryl group to an internal receiver domain, rather than to a separate RR protein. The phosphoryl group is then shuttled to histidine phosphotransferase (HPT) and subsequently to a terminal RR, which can evoke the desired response [PMID: 11934609, PMID: 11489844].
Signal transducing histidine kinases are the key elements in two-component signal transduction systems, which control complex processes such as the initiation of development in microorganisms [PMID: 8868347, PMID: 11406410]. Examples of histidine kinases are EnvZ, which plays a central role in osmoregulation [PMID: 10426948], and CheA, which plays a central role in the chemotaxis system [PMID: 9989504]. Histidine kinases usually have an N-terminal ligand-binding domain and a C-terminal kinase domain, but other domains may also be present. The kinase domain is responsible for the autophosphorylation of the histidine with ATP, the phosphotransfer from the kinase to an aspartate of the response regulator, and (with bifunctional enzymes) the phosphotransfer from aspartyl phosphate back to ADP or to water [PMID: 11145881]. The kinase core has a unique fold, distinct from that of the Ser/Thr/Tyr kinase superfamily.
HKs can be roughly divided into two classes: orthodox and hybrid kinases [PMID: 8029829, PMID: 1482126]. Most orthodox HKs, typified by the Escherichia coli EnvZ protein, function as periplasmic membrane receptors and have a signal peptide and transmembrane segment(s) that separate the protein into a periplasmic N-terminal sensing domain and a highly conserved cytoplasmic C-terminal kinase core. Members of this family, however, have an integral membrane sensor domain. Not all orthodox kinases are membrane bound, e.g., the nitrogen regulatory kinase NtrB (GlnL) is a soluble cytoplasmic HK [PMID: 10966457]. Hybrid kinases contain multiple phosphodonor and phosphoacceptor sites and use multi-step phospho-relay schemes instead of promoting a single phosphoryl transfer. In addition to the sensor domain and kinase core, they contain a CheY-like receiver domain and a His-containing phosphotransfer (HPt) domain.
SpoOB is a response regulator that responds to nutritional stress by inducing sporulation. SpoOB is a phosphotransferase that acts upon SpoOA using SpoOF as the phosphor-donor. Phosphorylated SpoOA ca activate sporulation-specific gene transcription [PMID: 1664534]. SpoOB has an alpha-beta core structure that resembles the histidine kinase fold, but lacks the kinase ATP-binding site [PMID: 9809070].
Domains with a similar structure to that found in SpoOB are present in other sensor proteins, such as malate kinase sensor MalK (EC:220.127.116.11), a member of a two-component regulatory system malK/malR that is involved in the activation of maeA, maeN and yflS in presence of malate [PMID: 12949160].
- SSF55890 (SSF55890)