Domain

Exotoxin A, middle domain (IPR015186)

Short name: Exotox-A_middle_dom

Domain relationships

None.

Description

This domain, found in Pseudomonas aeruginosa exotoxin A, is responsible for transmembrane targeting of the toxin, as well as transmembrane translocation of the catalytic domain into the cytoplasmic compartment. A furin cleavage site is present within the domain: cleavage generates a 37 kDa carboxy-terminal fragment, which includes the enzymatic domain, which is then is translocated into the cytoplasm. It adopts a helical structure, with six alpha-helices forming a bundle [PMID: 11734000].

Proteins contain this domain include exotoxin A from Pseudomonas aeruginosa and cholix toxin from Vibrio cholerae [PMID: 18276581]. They are NAD-dependent ADP-ribosyltransferases (ADPRT) that catalyses the transfer of the ADP-ribosyl moiety of oxidized NAD onto eukaryotic elongation factor 2 (eEF-2) thus arresting protein synthesis [PMID: 18276581].

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.
GENE3D
SUPERFAMILY
Pfam