Domain

Clathrin adaptor, phosphoinositide-binding, GAT-like (IPR014712)

Short name: Clathrin_Pinositid-bd_GAT-like

Domain relationships

None.

Description

Proteins synthesized on the ribosome and processed in the endoplasmic reticulum are transported from the Golgi apparatus to the trans-Golgi network (TGN), and from there via small carrier vesicles to their final destination compartment. These vesicles have specific coat proteins (such as clathrin or coatomer) that are important for cargo selection and direction of transport [PMID: 15261670]. Clathrin coats contain both clathrin (acts as a scaffold) and adaptor complexes that link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. The two major types of clathrin adaptor complexes are the heterotetrameric adaptor protein (AP) complexes, and the monomeric GGA (Golgi-localising, Gamma-adaptin ear domain homology, ARF-binding proteins) adaptors [PMID: 17449236, PMID: 11598180].

This entry represents a GAT-like domain with a 3-helical closed bundle structure, which is found in phosphoinositide-binding clathrin adaptor proteins: CALM (cathrin assembly myeloid leukaemia protein) and AP180 (adaptor protein 180). Clathrin-mediated endocytosis plays an important role in retrieving synaptic vesicles from the plasma membrane following exocytosis, and requires the action of CALM or AP180 [PMID: 11239400, PMID: 11161218]. These proteins act to restrict the size of the clathrin-coated vesicles. The phosphoinositide-binding domain regulates the clathrin assembly activity of these proteins. Phosphoinositides control the timing and localization of endocytic membrane trafficking by recruiting adaptors and other components of the transport machinery, thereby being part of a coincidence detection system in adaptor-mediated vesicle transport [PMID: 16246100].

GO terms

Biological Process

GO:0048268 clathrin coat assembly

Molecular Function

GO:0005545 1-phosphatidylinositol binding
GO:0030276 clathrin binding

Cellular Component

GO:0030118 clathrin coat

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.
GENE3D