Domain

RAD50, zinc hook (IPR013134)

Short name: Zn_hook_RAD50

Domain relationships

None.

Description

The MRN complex (Mre11-Rad50-Nbs1) plays an important role in many DNA metabolic events that involve DNA double-stranded breaks. MRN is one of the first factors to be localised to DNA lesions where it might have a structural role by tethering and stabilising broken chromosomes [PMID: 12949583, PMID: 11741547].

Rad50 is a split ABC-type ATPase; its centre contains a long heptad repeat that folds into an antiparallel coiled coil, bringing the N-terminal (Walker A) and the C-terminal (Walker B) domains in close proximity [PMID: 12152085]. The apex of the coiled coil contains a dimerization interface, a conserved Cys-X-X-Cys motif in a hook-shaped domain that dimerizes with a second hook domain via cysteine-mediated zinc ion coordination. This zinc dependent dimerization event allows the formation of a complex that has appropriate lengths and conformational proporties to link sister chromatids in homologous recombination and DNA ends in non-homologous end-joining.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.
PROSITE profiles
Pfam