Pathways & interactions
ADP-ribosylarginine hydrolase (IPR012108)
Short name: ADP-ribosylarg_hydro
Overlapping homologous superfamilies
- ADP-ribosylation/Crystallin J1 superfamily (IPR036705)
- ADP-ribosylation/Crystallin J1 (IPR005502)
- ADP-ribosylarginine hydrolase (IPR012108)
This enzyme catalyzes the reverse reaction of mono-ADP-ribosylation by removing ADP-ribose from arginine residues in ADP-ribosylated proteins. NAD:arginine ADP-ribosyltransferases and ADP-ribosylarginine hydrolases catalyse opposing arms of the putative ADP-ribosylation cycle.
ADP-ribosylarginine hydrolases from mammalian tissues and Rhodospirillum rubrum exhibit three regions of similarity in their deduced amino acid sequence. The conserved vicinal aspartates 60 and 61 in rat ADP-ribosylarginine hydrolase are critical for catalytic activity, but not for high affinity binding of the substrate analogue, ADP-ribose [PMID: 10358013].
ADP-ribosylarginine hydrolases in different organisms also differ in their dithiothreitol (DTT) requirements. Cysteine 108 in rat hydrolase plays a critical role in DTT dependence and may be important in immunoreactivity [PMID: 8349667].
- PIRSF016939 (ADP_ribslarg_hdr)