Methane/phenol monooxygenase, hydroxylase component (IPR012078)

Short name: MP_mOase_hydro

Overlapping homologous superfamilies

Family relationships


This group represents the hydroxylase component of aromatic and alkene monooxygenases such as methane monooxygenase (EC: phenol 2-monooxygenase (EC:, toluene-4-monooxygenase (EC: from Pseudomonas mendocina, and alkene monooxygenase (EC: from Xanthobacter autotrophicus. Aromatic and alkene monooxygenase hydroxylases (AAMH) are composed of two copies each of three subunits (alpha 2 beta 2 gamma 2), and all three subunits are almost completely alpha-helical, with the exception of two beta hairpin structures in the alpha subunit. The active site of each alpha subunit contains one dinuclear iron centre, housed in a four-helix bundle [PMID: 9329079].

Methane monooxygenase is a multicomponent enzyme found in methanotrophic bacteria that catalyzes the hydroxylation of methane and higher alkenes (as large as octane). Phenol monooxygenase, found in a diverse group of bacteria, catalyses the hydroxylation of phenol, chloro- and methyl-phenol and naphthol. Both enzyme systems consist of three components: the hydroxylase, a coupling protein and a reductase [PMID: 11456616]. The toluene-4-monooxygenase multicomponent enzyme system catalyzes the O2- and NADH-dependent hydroxylation of toluene to form p-cresol [PMID: 11297417]. The alkene monooxygenase system catalyzes the O2- and NADH-dependent epoxidation of short chain (C2 to C6) alkenes to their corresponding epoxides [PMID: 9312093].

Please see the following relevant references: [PMID: 7753034, PMID: 15544, PMID: 810352, PMID: 15379538].

GO terms

Biological Process

GO:0055114 oxidation-reduction process

Molecular Function

GO:0016709 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen

Cellular Component

No terms assigned in this category.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.