Domain

Winged helix-turn-helix DNA-binding domain (IPR011991)

Short name: WHTH_DNA-bd_dom

Domain relationships

Description

Winged helix DNA-binding proteins share a related winged helix-turn-helix DNA-binding motif, where the "wings", or loops, are small beta-sheets. The winged helix motif consists of two wings (W1, W2), three alpha helices (H1, H2, H3) and three beta-sheets (S1, S2, S3) arranged in the order H1-S1-H2-H3-S2-W1-S3-W2 [PMID: 10679470]. The DNA-recognition helix makes sequence-specific DNA contacts with the major groove of DNA, while the wings make different DNA contacts, often with the minor groove or the backbone of DNA. Several winged-helix proteins display an exposed patch of hydrophobic residues thought to mediate protein-protein interactions.

Many different proteins with diverse biological functions contain a winged helix DNA-binding domain, including transcriptional repressors such as biotin repressor, LexA repressor and the arginine repressor [PMID: 1409631]; transcription factors such as the hepatocyte nuclear factor-3 proteins involved in cell differentiation, heat-shock transcription factor, and the general transcription factors TFIIE and TFIIF [PMID: 8248124, PMID: 11292844]; helicases such as RuvB that promotes branch migration at the Holliday junction, and CDC6 in the pre-replication complex [PMID: 12408833, PMID: 11030343]; endonucleases such as FokI and TnsA [PMID: 9214510]; histones; and Mu transposase, where the flexible wing of the enhancer-binding domain is essential for efficient transposition [PMID: 8577730].

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.
GENE3D
Pfam