Domain

Gamma-adaptin ear (GAE) domain (IPR008153)

Short name: GAE_dom

Overlapping homologous superfamilies

Domain relationships

Description

The adaptor proteins AP-1 and GGA (Golgi-localized, gamma ear-containing, ADP- ribosylation factor (ARF)-binding proteins) regulate membrane traffic between the trans-Golgi network (TGN) and endosome/lysosomes through ARF-regulated membrane association, recognition of sorting signals, and recruitment of clathrin and accessory proteins. The gamma-adaptin ear (GAE) domain is a C- terminal appendage or ear of about 120 residues, which is found in gamma- adaptins, the heavy subunits of the AP-1 complex, and in GGAs. The GAE domain, which is found in associated with other domains such as VHS, coiled-coils and GAT, is involved in the recruitment of accessory proteins, such as gamma-synergin, Rababptin-5, Eps15 and cyclin G-associated kinase, which modulate the functions of GAE domain containing proteins in the membrane trafficking events [PMID: 10747088, PMID: 10702286, PMID: 12042876, PMID: 12176391].

The resolution of the 3D-structure of the human gamma-adaptin GAE domain shows that it forms an immunoglobulin-like beta-sandwich fold composed of eight beta-strands with two short alpha-helices. The topology of the entire GAE domain is similar to those of the N-terminal subdomains in the alpha- and beta-adaptin ear domains of the AP-2 complex. However, the GAE domain has very low sequence identity and homology to the N-terminal immunoglobulin-like subdomains of the alpha and beta ear domains. The binding site for the accessory proteins has been located to a shallow hydrophobic trough surrounded by charged (mainly basic) residues [PMID: 12042876, PMID: 12176391].

This entry represents the entire GAE domain.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.
PROSITE profiles